Effect of Gonadotropin Hormones on Viability, Morphology and In Vitro Maturation of Sheep Oocytes
DOI:
https://doi.org/10.51699/ijbea.v3i3.86Keywords:
In Vitro Maturation (IVM), Gonadotropin, FSH, Immature Oocyte, Sheep, HcgAbstract
The study investigates the impact of gonadotropin hormones on the viability, morphology, and in vitro maturation of sheep oocytes, addressing a knowledge gap in optimizing culture conditions for oocyte maturation. Using a controlled experiment, immature sheep oocytes were divided into groups with varying concentrations of hCG and PMSG hormones and incubated under specific conditions. The findings revealed that the addition of gonadotropins significantly increased oocyte viability, with the highest viability and normal morphology observed in the group with the highest hormone concentration. These results underscore the critical role of gonadotropins in enhancing oocyte maturation, with implications for improving in vitro fertilization techniques in mammals.
References
Ferreira, E. M., Vireque, A. A., Adona, P. R., Meirelles, F. V., Ferriani, R. A., and Navarro, P. A. D. Cytoplasmic maturation of bovine oocytes: structural and biochemical modifications and acquisition of developmental competence. Theriogenology. 2009; 71, 836–848. doi: 10.1016/j.theriogenology.2008.10.023
Gilchrist, R. B. Recent insights into oocyte–follicle cell interactions provide opportunities for the development of new approaches to in vitro maturation. Reprod. Fertil. Dev.2011; 23, 23–31. doi: 10.1071/RD10225
Pincus G, Enzmann EV.The comparative behavior of mammalian eggs in vivo and in vitro: I. The activation of ovarian eggs. The Journal of Experimental Medicine. 1935;62:665-675
Trounson A, Wood C, Kausche A. In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertility and Sterility. 1994;62:353-362.
Zhu, J., Moawad, A. R., Wang, C. Y., Li, H. F., Ren, H. F., & Dai, Y. F. Advances in in vitro production of sheep embryos. International Journal of Veterinary Science and Medicine,2018; 6(2), S15-S26. doi: 10.1016/j.ijvsm.2018.02.003
Del Collado, M., Saraiva, N. Z., Lopes, F. L., Gaspar, R. C., Padilha, L. C., Costa, R. R., Rossi, G. F., Vantini, R., & Garcia, J. M. Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos. Reproduction, Fertility and Development, 2015; 28(11), 1721-1732. doi: 10.1071/RD15067
Dumesic DA, Meldrum DR, Katz-Jaffe MG, Krisher RL, Schoolcraft WB. Oocyte environment: Follicular fluid and cumulus cells are critical for oocyte health. Fertil Steril. 2015;103:303–16.
Lee, H. S., Seo, Y. I., Yin, X. J., Cho, S. G., Lee, S. S., Kim, N. H., Cho, S. K., & Kong, I. K. Effect of follicle stimulation hormone and luteinizing hormone on cumulus cell expansion and in vitro nuclear maturation of canine oocytes. Reproduction in Domestic Animals, 2007; 42(6), 561-565. doi: 10.1111/j.1439-0531.2006.00818.x
Xiao, X., Zi, X. D., Niu, H. R., Xiong, X. R., Zhong, J. C., Li, J., Wang, L., & Wang, Y. Effect of addition of FSH, LH and proteasome inhibitor MG132 to in vitro maturation medium on the developmental competence of yak (Bos grunniens) oocytes. Reproductive Biology and Endocrinology, 2014; 12(30), 1-7. doi: 10.1186/1477-7827-12-30
Coelho, L. A. D., Esper, C. R., Alvarez, R. H., Vantine, R., & Almeida, I. L., Jr. Produção in vitro de embriões bovinos: utilização de diferentes fontes de gonadotrofinas na maturação dos oócitos. Revista Brasileira de Zootecnia, 2002; 31(3), 1117-1121. doi: 10.1590/S1516- 35982002000500007
De Los Reyes, M., De Lange, J., Miranda, P., Palominos, J., & Barros, C. of human chorionic gonadotrophin supplementation during different culture periods on in vitro maturation of canine oocytes. Theriogenology, 2005; 64(1), 1-11. doi: 10.1016/j.theriogenology.2004.11.008
Mingoti, G. Z., Garcia, J. M., & Rosa-e- Silva, A. A. M. Steroidogenesis in cumulus cells of bovine cumulus oocyte-complexes matured in vitro with BSA and different concentrations of steroids. Animal Reproduction Science, 2002; 69(3-4), 175-186. doi: 10.1016/S0378- 4320(01)00187-7
De Felici M and Siracusa G. Spontaneous hardening of the zonapellucida of mouse oocytes duringin vitro culture. Gamete Res. 1982; 6: 107-113.
Bols PEJ, Jorsen EPA, Goovaerts IGF, et al. High throughput non-invassive oocyte quality assessment: the search continues. Animal Reproduction. 2012;9(3): 420-425.
Abd-Allah SM. Effects of storage conditions of dromedary camel ovaries on the morphology, viability and development of antral follicular oocytes. Anim. Reprod. 2010; 7(2):65-69.
Downs SM and Mastropolo AM. Culture conditions affect meiotic regulation in cumulus-cell enclosed mouse oocytes Molecular Reproduction and Development. 1997; 46:551-566.
Goud PT, Goud AP, Qian C,et al. In vitro maturation of human germinal vesicle stage oocytes: role of cumulus cells and epidermal growth factor in the culture medium Human Reproduction. 1998; 13(6): 1638-1644.
Sugimura, S., Yamanouchi, T., Palmerini, M. G., Hashiyada, Y., Imai, K., & Gilchrist, R. B. J. Effect of pre-in vitro maturation with cAMP modulators on the acquisition of oocyte developmental competence in cattle. Journal of Reproduction, 2018; 64(3), 233-241. doi: 10.1262/jrd.2018-009
Pogrmic-Majkic, K., Samardzija, D., Stojkov- Mimic, N., Vukosavljevic, J., Trninic Pjevic, A., Kopitovic, V., & Andric, N. A. Atrazine suppresses FSH-induced steroidogenesis and LH-dependent expression of ovulatory genes through PDE cAMP signaling pathway in human cumulus granulosa. Molecular and Cellular Endocrinology, 2018; 461(1), 79-88. doi: 10.1016/j.mce.2017.08.015
Cantanhêde, F. C. Influência do FSH sobre os aspectos celulares e moleculares na maturação in vitro de complexo’s cumulus-ovócitos de bovinos. Tese de Doutorado, Universidade Federal Rural de Pernambuco, PE, Brasil. 2018; http:// www.tede2.ufrpe.br:8080/tede2/handle/ tede2/7507
Moor RM andTrounson AO. Hormonal and follicular factors affecting maturation of sheep oocytes in vitro and their subsequent development capacity. J ReprodFertil. 1997;49:101-9.
Downs SM, Danie SAJ and Eppig JJ. Induction of maturation in cumulus cell-enclosed mouse oocytes by follicle-stimulating hormone and epidermal growth factor - evidence for a positive stimulus of somatic cell origin. J. Exp. Zool.1988; 245; 86-96.
Turathum, B., Gao, E. M., & Chian, R. C. The function of cumulus cells in oocyte growth and maturation and in subsequent ovulation and fertilization. Cells, 2021; 10(9), 2292-2293. doi: 10.3390/cells10092292
