On the Results of Genotyping of Sheep of Jaidar, Karakul, Gissar Breeds by Locus of Calpastatin Gene
DOI:
https://doi.org/10.51699/ijbea.v3i5.205Keywords:
breeds of sheep jaidary, karakul, gissra, sheep genotyping, PCR-RFLP analysis, calpastatin gene, CAST, gene equilibriumAbstract
The article presents the results of genotyping of three local breeds of the Republic of Uzbekistan, jaidary (n-14), karakul (n-17) and hissar (n-18) barracks by the locus of the meat productivity gene, calpastatin (CAST). According to the results of the study, genetic polymorphism was detected in all three breeds, the homozygous MM genotype and the heterozygous MN genotype were determined, but another homozygous NN was not detected in the animals under study, a violation of gene balance is observed, and the M allele (0.82) is predominantly found. The authors of the work used the PCR-RFLP method for genotyping, identification of the CAST gene alleles was carried out using the MspI restriction enzyme, the length of the PCR product was 565 bp, after restriction of the amplification, depending on the genotype of the animals, three fragments were found: 565 bp, 306 bp, 259 bp.
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